Bridging Spatial Omics: DBiTplus Maps Transcriptomes and Proteomes on a Single Tissue

The DBiTplus method introduces a cutting-edge spatial omics approach that unites sequencing-based spatial transcriptomics with image-based protein profiling on the same tissue section. By combining in situ reverse transcription, microfluidic spatial barcoding, and high-plex protein imaging (CODEX), DBiTplus enables single-cell resolution analysis of both transcriptomic and proteomic landscapes. This integration allows precise cell typing and genome-scale exploration of biological pathways. The method was validated on diverse tissue types, including mouse embryos, human lymph nodes, and lymphoma tissues, showcasing its potential to unravel complex cellular heterogeneity and disease mechanisms. With its unified experimental and computational framework, DBiTplus paves the way for advanced spatially resolved single-cell studies.