Tristate Logic Enhances Genetic Computation for Precision Medicine

Traditional biocomputation relies on binary logic, limiting the complexity of gene circuit design. In a breakthrough approach, researchers developed a tristate-based logic synthesis (TriLoS) framework, introducing genetic tristate buffers as fundamental processing units instead of conventional logic gates. This enables multi-layered gene networks to efficiently execute complex Boolean calculations within single cells, expanding the engineering space for genetic computation. The study demonstrates cellular-level full adder and full subtractor operations, paving the way for programmable cell-based therapies. By embedding adjustable and disease-specific drug secretion logics, this innovation brings synthetic biology closer to precision medicine, allowing for customized therapeutic responses tailored to individual patient needs. This advance could revolutionize genetic circuits, bio-computation, and in vivo therapeutic applications.